BOPI 07 BR/2023 REPERTOIRE NUMERIQUE 41 (11) 21074 (51) A01H 1/04 (2018.01); A01H 1/08 (2018.01); A01H 6/46 (2018.01); C07K 14/415 (2018.01); C12N 15/29 (2018.01); C12N 15/82 (2018.01) ; C12N 9/22 (2018.01) (21) 1202200508 - PCT/US2021/036605 (22) 09/06/2021 (30) US n° 63/036,910 du 09/06/2020; US n° 63/036,902 du 09/06/2020 (54) Heterozygous CENH3 monocots and methods of use thereof for haploid induction and simultaneous genome editing. (72) DAWE, R., Kelly (US) et JACKSON, David (US) (73) 1-UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., 210 S Jackson Street, 110 Terrell Hall, ATHENS, GA 30602 (US) et 2-COLD SPRING HARBOR LABORATORY, 1 Bungtown Road, COLD SPRING HARBOR, NY 11724 (US) (74) S.C.P AKKUM, AKKUM & Associates, n° 1777, Rue 6.261, Auditorium Jean Paul II, Quartier Mbankolo, B.P. 4966, YAOUNDE (CM). (57) Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally passthrough gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENII3 protein. In some embodiments, the allele encoding nonfunctional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant's genome are also provided. Fig. 1A ;
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